Douglas W. Kline
Ph.D., University of California, Davis
Molecular Biology Lab
Scholarly, Creative & Professional Activities
We investigate some of the mechanisms responsible for the normal production of reproductive cells in the ovary.Â Despite significant advances in the study of oogenesis, there are major gaps in our understanding of how mammalian meiosis is regulated by signaling systems. Normal oocyte maturation is necessary for the production of viable eggs and embryos. We are identifying and studying the combination of interacting proteins that are responsible for initiating and regulating oocyte maturation. Clarifying the signaling pathways and protein interactions will enhance our understanding of mammalian oogenesis and provide fundamental information about cell division.
De, S. and Kline, D. 2013. Evidence for the requirement of 14-3-3eta (YWHAH) in meiotic spindle assembly during mouse oocyte maturation. BMC Developmental Biology. 13,10.
De, S., Marcinkiewicz, J., Vijayaraghavan, S., and Kline, D. 2012. Expression of protein 14-3-3 isoforms in mouse oocytes, eggs and ovarian follicular development. BMC Res Notes. 5(1), 57.
Pawan Puri, P., Acker-Palmer, A., Stahler, Chen, Y., Kline, D., and Vijayaraghavan, S. 2011. Identification of testis 14-3-3 binding proteins by tandem affinity purification. Spermatogenesis, 1(4), 354-365.
Puri, P, Myers, K. Kline, D. and Vijayaraghavan, S. 2008 Proteomic analysis of bovine sperm YWHA binding partners identify proteins involved in signaling and metabolism. 2008. Biology of Reproduction, 7, 1183-1191.
Snow, A.J., Puri, P., Acker-Palmer, A., Bouwmeester, T., Vijayaraghavan, S., and Kline, D. 2008. Phosphorylation-dependent interaction of tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein (YWHA) with PADI6 following oocyte maturation in mice. Biology of Reproduction. 79, 33-347.
Chakrabarti, R., Kline, D., Lu, J., Orth, J., Pilder, S., and Vijayaraghavan, S. 2007. Analysis of Ppp1cc-Null Mice Suggests a Role for PP1gamma2 in Sperm Morphogenesis. Biology of Reproduction 76, 992-1001.
Kline, D., Mehlmann, L., Fox, C., and Terasaki, M. 1999. The cortical endoplasmic reticulum (ER) of the mouse egg: Localization of ER clusters in relation to the generation of repetitive calcium waves. Developmental Biology 215, 431-442.
Wolosker, H., Kline, D., Bian, Y. Blackshaw, S., Cameron, A.M., Fralich, T.J. Schnaar, R.L. and Snyder, S.H. 1998. Molecularly cloned mammalian glucosamine-6-phosphate deaminase localizes to transporting epithelium and lacks oscillin activity. FASEB Journal 12, 91-99.
Mehlmann, L.M., Mikoshiba, K., and Kline, D. 1996. Redistribution and increase in cortical inositol 1,4,5-trisphosphate receptors after meiotic maturation of the mouse oocyte. Developmental Biology 180, 489-498.
Kline, D. and Zagray, J. 1995. Absence of an intracellular pH change following fertilization of the mouse egg. Zygote 3, 305-311.
Mehlmann, L.M., Terasaki, M., Jaffe, L.A., and Kline, D. 1995. Reorganization of the endoplasmic reticulum during meiotic maturation on the mouse oocyte. Developmental Biology 170, 607-615.
Mehlmann, L.M., and Kline, D. 1994. Regulation of intracellular calcium in the mouse egg: Calcium release in response to sperm or inositol trisphosphate is enhanced after meiotic maturation. Biology of Reproduction 51, 1088-1098.
Kline, D., and Stewart-Savage, J. 1994. The timing of cortical granule fusion, contents dispersal and endocytosis during fertilization of the hamster egg: An electrophysiological and histochemical study. Developmental Biology 162, 277-287.
Kline, J.T. and Kline, D. 1994. Regulation of intracellular calcium in the mouse egg: Evidence for inositol trisphosphate-induced calcium release, but not calcium-induced calcium release. Biology of Reproduction 50, 193-203.
Kline, D. and Kline, J.T. 1992. Repetitive calcium transients and the role of calcium in exocytosis and cell cycle activation in the mouse egg. Developmental Biology 149, 80-92.
Kline, D. and Kline, J.T. 1992. Thapsigargin activates a calcium influx pathway in the unfertilized mouse egg and suppresses repetitive calcium transients in the fertilized egg. Journal of Biological Chemistry 267, 7624-17630.
Kline D., and Nuccitelli, R. 1985. The wave of activation current in the Xenopus egg. Developmental Biology 111, 471-487.
Kline, D., Jaffe, L.A., and Tucker, R.P. 1985. The fertilization potential and polyspermy prevention in the egg of the nemertean, Cerebratulus lacteus. Journal of Experimental Zoology 236, 45-52.
Kline, D. 1988. Calcium-dependent events at fertilization of the frog egg: Injection of a calcium buffer blocks ion channel opening, exocytosis, and formation of pronuclei. Developmental Biology 126, 346-361.
Department of Biological Sciences