Research Facilities and Instrumentation
Interdisciplinary collaborations among the science departments has produced a set of outstanding and modern core research facilities available to faculty and students on the Kent State campus. Departmental facilities provide additional resources and instrumentation for both research and graduate education.These independent facilities can be found at the following Kent Web sites: Department of Chemistry, Department of Biological Sciences, Department of Computer Science and Department of Physics.
Below is a summary of instrumentation contained within core facilities collaboratively maintained by the School of Biomedical Sciences, the Department of Biological Sciences, the Department of Chemistry and the Department of Computer Science.
Imaging Core Facility
This core facility contains equipment and instrumentation for fluorescence and confocal microscopy. The laboratory is outfitted for working with cell and tissue cultures and contains requisite equipment including incubators, -70 freezer, BioRad electrophoresis unit, Olympus FV300 confocal microscope, Olympus FV500 confocal microscope, and Olympus IX70 fluorescence microscope. A second companion laboratory contains a Bruker IFS 66 FTIR with Hyperion Focal Plane Array Microscope for acquisition of infrared images with 5 micrometer resolution, a Bruker Tensor spectrometer with Confocheck ATR unit for protein structure determination, and a Bruker Equinox spectrometer with XA511 external reflection unit for the in situ characterization of monolayers at the air/water interface.
A third companion laboratory contains a Varian Unity/Inova 500 MHz NMR spectrometer is equipped with a microimaging kit, which includes the Varian 5mm XYZ triple resonance probe and Performa XYZ PFG module. The equipment allows to acquire 3D images of small objects (about 10 mm long and 4mm diameter) with up to 20 micron resolution using NMR technology.
Genomics Core Facility
Our genomics core is partially complete and currently contains an Applied Biosystems 7000 real-time PCR, Arcturus Autopix Laser capture system, Affymetrix microarray station. The Department of Chemistry maintains the Advanced Recombinant DNA Laboratory and the Protein/Enzyme Laboratory, each with equipment applicable to genomic investigations. We are in the process of purchasing a Typhoon variable mode imager for fluorescent microarray imaging and analysis.
Proteomic Core Facility
We are in the process of substantially upgrading instrumentation necessary for assessment of the proteome. In addition to equipment included in the core facilities detailed at the above websites, our Proteomic Core Facility will substantially improve with the impending purchase of a new MALDI/TOF and/or SELDI instrument(s) and additional instrumentation for protein separations. Furthermore, the Department of Chemistry maintains outstanding facilities for protein separation and chemistry, which can be found at the website noted above.
Computational Core Facility
The science units at Kent State have secured resources for a dedicated Gbit+ science network. This network is designed for the movement of large data sets, including those derived from biomedical investigations, especially those involving data from imaging techniques such as confocal microscopy and MRI. Kent has nationally recognized expertise in the use of cluster computing for analysis of large data sets and several computer clusters dedicated to interdisciplinary efforts in the biomedical sciences are available for data visualization and analysis. These and related resources including a wide variety of software offers biomedical researchers a modern high-performance computational environment for a variety of types of analyses. The network, to be lit in April 2004 and will shortly thereafter be connected to access to the Ohio's Bit+ Third Frontier Network and Internet 2.
3D Immersive Development Facility and Classroom
The School and science departments have developed a strong initiative in 3D visualization, especially of biological materials but also of lyotropic liquid crystals. We currently have several immersive systems (Fakespace immersadesk, Vrex display) for 3D display of large data sets. This spring, we will have completed a 3D immersive classroom consisting of a 7x8 Fakespace Powerwall. These systems are in increasing use as tools for visualization of multidimensional structures, such as cells, proteins, membranes, etc., and provide valuable means for evaluating the importance of spatial properties in determining function. In conjunction with the computational resources available at Kent, this facility is unique in the State of Ohio and forms both a research and educational tool for the multiple scientific disciplines.
Facilities at Participant Institutions: