Aleisha Moore
Biography
I am interested in defining and understanding the neuronal network regulating gonadotropin-releasing hormone (GnRH) neurons, the final output cells controlling fertility. In particular, my work involves determining whether changes in the regulation of GnRH neurons by a population of cells that co-express the neuropeptides Kisspeptin, Neurokinin B and Dynorphin (KNDy neurons) underlies the development of polycystic ovarian syndrome (PCOS), the most common cause of female infertility. My research explores the role of KNDy neurons in the pathogenesis of PCOS using a preclinical mouse model of the syndrome combined with a range of anatomical and functional tools that permit the study of complicated neuroendocrine networks, including viral-mediated tract-tracing, fluorescent in situ hybridization, immunolabeling, steroid hormone feedback protocols, ELISAs, chemogenetics, optogenetics, calcium imaging and electron, light sheet, confocal and miniature microscopy.
Biography
Research Interests:
I am interested in understanding how fertility is controlled by a population of cells in the brain that co-express the neuropeptides Kisspeptin, Neurokinin B and Dynorphin (KNDy) neurons. Currently, my primary focus is investigating whether alterations to KNDy cells lead to the development of polycystic ovarian syndrome (PCOS), the most common cause of female infertility worldwide. For up to date info on research and opportunities in my lab, please contact me by email (amoor149@kent.edu) or visit my lab website (www.reproneuro.com).
Research Methods:
My research explores the role of KNDy neurons in the pathogenesis of PCOS using a preclinical model of the syndrome combined with a range of anatomical and functional tools that permit the study of complicated neuroendocrine networks, including viral-mediated tract-tracing, fluorescent in situ hybridization, immunohistochemistry, steroid hormone feedback protocols, ELISAs, chemogenetics, optogenetics, calcium imaging and electron, light sheet, confocal and miniature microscopy
Publications:
AM Moore. GnRH pulse generation in rodents: Time to terminate the role of dynorphin? Endocrinology. doi: 10.1210/endocr/bqad005.
BB Jamieson, AM Moore, DB Lohr, SX Thomas, LM Coolen, MN Lehman, RE Campbell, R Piet. 2022. Prenatal androgen treatment impairs the suprachiasmatic nucleus arginine-vasopressin to kisspeptin neuron circuit in female mice. Frontiers in Endocrinology. doi: 10.3389/fendo.2022.951344
AM Moore. 2022. Impaired steroid hormone feedback in polycystic ovary syndrome: Evidence from preclinical models for abnormalities within central circuits controlling fertility. Clinical Endocrinology. March 29, DOI:10.1111/cen.14711
AM Moore, LM Coolen, MN Lehman. 2022. In vivo calcium imaging of the GnRH pulse generator reveals a temporal order of activation and synchronization during each pulse. 2022. Proc. Natl. Acad. Sci (PNAS). Feb 8;119(6)e2117767119. doi: 10.1073/pnas.2117767119.
AM Moore, DB Lohr, LM Coolen, MN Lehman. Prenatal androgen exposure alters KNDy neurons and their afferent network in a mouse model of polycystic ovarian syndrome. 2021 Nov 1. Endocrinology. 162(11):bqab158 doi: 10.1210/endocr/bqab158.
AM Moore, LM Coolen, MN Lehman. Kisspeptin/Neurokinin B/Dynorphin (KNDy) cells as integrators of diverse internal and external cues: evidence from viral-based monosynaptic tract-tracing in mice. 2019 Oct 14. Scientific Reports, 9(1):14768. doi:10.1038/s41598-019-51201-0
DT Porter, AM Moore, JA Cobern, V Padmanabhan, RL Goodman, LM Coolen, MN Lehman. Prenatal Testosterone Exposure Alters GABAergic Synaptic Inputs to GnRH and KNDy Neurons in a Sheep Model of Polycystic Ovarian Syndrome. 2019 Nov 1. Endocrinology, 160(11):2529-2542. doi: 10.1210/en.2019-00137.
AM Moore, LM Coolen, DT Porter, RL Goodman, MN Lehman. KNDy Cells Revisited. 2018 Sep 1. Endocrinology, 159(9):3219-3234. doi: 10.1210/en.2018-00389.
AM Moore, G Abbott, J Mair, RE Campbell. Mapping GABA and glutamate inputs to gonadotropin-releasing hormone neurones in male and female mice. 2018 Nov 29. Journal of Neuroendocrinology, 30 (12), e12657. doi: 10.1111/jne.12657.
AM Moore, M Prescott, K Czieselsky, E Desroziers, SH Yip, RE Campbell, AE Herbison. 2018 Synaptic innervation of the GnRH neuron distal dendron in female mice. Endocrinology. 159(9):3200-3208. doi: 10.1210/en.2018-00505.
MN Lehman, LM Coolen, RA Steiner, G Neal-Perry, L Wang, SM Moenter, AM Moore, R Goodman, S Hwa Yeo, S Padilla, A Kauffman, J Garcia, M Kelly, J Clarkson, S Radovick, A Babwah, S Tellez, M Tena-Sempere, A Comninos, S Seminara, W Dhillo, J Levine, E Terasawa, A Negron, A Herbison. 2018. The 3rd World Conference on Kisspeptin, “Kisspeptin 2017: Brain and Beyond”: Unsolved questions, challenges and future directions for the field. Journal of Neuroendocrinology. 2018 Apr 14:e12600. doi: 10.1111/jne.12600
AM Moore, KA Lucas, LM Coolen and MN Lehman. 2018. Three-dimensional imaging of KNDy neurons in the mammalian brain using optical tissue clearing and immunocytochemistry. Scientific Reports. 8(1):2242. doi: 10.1038/s41598-018-20563-2.
AM Moore and RE Campbell. 2017 Polycystic ovary syndrome: Understanding the role of the brain. Frontiers in Neuroendocrinology. 46:1-14.
AM Moore and RE Campbell. 2015. The neuroendocrine genesis of polycystic ovarian syndrome: A role for arcuate nucleus GABA neurons. J. Steroid Biochem. Mol. Biol 160:106-117.
AM Moore, M Prescott, CJ Marshall, SH Yip, RE Campbell. 2015. Enhancement of a robust arcuate GABAergic input to gonadotropin-releasing hormone neurons in a mouse model of polycystic ovarian syndrome. Proc. Natl. Acad. Sci. (PNAS) U.S.A 112.2, 596-601.
AM Moore, Prescott, M. Campbell, RE. 2013. Investigating estradiol negative and positive feedback in a prenatal androgen-induced mouse model of polycystic ovarian syndrome. Endocrinology 154, 796-806.
Affiliations
Brain Health Research Institute (BHRI), the Pan-American Neuroendocrine Society (PANS)
Education:
2015, PhD in Neuroscience, University of Otago.
Education
Publications
- 1. AM Moore. GnRH pulse generation in rodents: Time to terminate the role of dynorphin? Endocrinology. doi: 10.1210/endocr/bqad005.
- 2. BB Jamieson, AM Moore, DB Lohr, SX Thomas, LM Coolen, MN Lehman, RE Campbell, R Piet. 2022. Prenatal androgen treatment impairs the suprachiasmatic nucleus arginine-vasopressin to kisspeptin neuron circuit in female mice. Frontiers in Endocrinology. do
- 3. AM Moore. 2022. Impaired steroid hormone feedback in polycystic ovary syndrome: Evidence from preclinical models for abnormalities within central circuits controlling fertility. Clinical Endocrinology. March 29, DOI:10.1111/cen.14711
- 4. AM Moore, LM Coolen, MN Lehman. 2022. In vivo calcium imaging of the GnRH pulse generator reveals a temporal order of activation and synchronization during each pulse. 2022. Proc. Natl. Acad. Sci (PNAS). Feb 8;119(6)e2117767119. doi: 10.1073/pnas.21177
- 5. AM Moore, DL Moore, LM Coolen, MN Lehman. 2021. Prenatal androgen exposure alters KNDy neurons and their afferent network in a mouse model of polycystic ovarian syndrome. Nov. 1 Endocrinology. doi:10.1210/endocr/bqab158
- 6. AM Moore, LM Coolen, MN Lehman. Kisspeptin/Neurokinin B/Dynorphin (KNDy) cells as integrators of diverse internal and external cues: evidence from viral-based monosynaptic tract-tracing in mice. 2019 Oct 14. Scientific Reports, 9(1):14768. doi:10.1038/
- 7. DT Porter, AM Moore, JA Cobern, V Padmanabhan, RL Goodman, LM Coolen, MN Lehman. Prenatal Testosterone Exposure Alters GABAergic Synaptic Inputs to GnRH and KNDy Neurons in a Sheep Model of Polycystic Ovarian Syndrome. 2019 Nov 1. Endocrinology, 160(11
- 8. AM Moore, LM Coolen, DT Porter, RL Goodman, MN Lehman. KNDy Cells Revisited. 2018 Sep 1. Endocrinology, 159(9):3219-3234. doi: 10.1210/en.2018-00389.
- 9. AM Moore, G Abbott, J Mair, RE Campbell. Mapping GABA and glutamate inputs to gonadotrophin-releasing hormone neurones in male and female mice. 2018 Nov 29. Journal of Neuroendocrinology, 30 (12), e12657. doi: 10.1111/jne.12657.
- 10. AM Moore, M Prescott, K Czieselsky, E Desroziers, SH Yip, RE Campbell, AE Herbison. 2018 Synaptic innervation of the GnRH neuron distal dendron in female mice. Endocrinology. 159(9):3200-3208. doi: 10.1210/en.2018-00505.
- 11. MN Lehman, LM Coolen, RA Steiner, G Neal-Perry, L Wang, SM Moenter, AM Moore, R Goodman, S Hwa-Yeo, S Padilla, A Kauffman, J Garcia, M Kelly, J Clarkson, S Radovick, A Babwah, S Tellez, M Tena-Sempere, A Comninos, S Seminara, W Dhillo, J Levine, E Ter
- 12. AM Moore, KA Lucas, LM Coolen and MN Lehman. 2018. Three-dimensional imaging of KNDy neurons in the mammalian brain using optical tissue clearing and immunocytochemistry. Scientific Reports. 8(1):2242. doi: 10.1038/s41598-018-20563-2.
- 13. AM Moore and RE Campbell. 2017 Polycystic ovary syndrome: Understanding the role of the brain. Frontiers in Neuroendocrinology. 46:1-14.
- 14. AM Moore and RE Campbell. 2015. The neuroendocrine genesis of polycystic ovarian syndrome: A role for arcuate nucleus GABA neurons. J. Steroid Biochem. Mol. Biol 160:106-117.
- 15. AM Moore, M Prescott, CJ Marshall, SH Yip, RE Campbell. 2015. Enhancement of a robust arcuate GABAergic input to gonadotropin-releasing hormone neurons in a mouse model of polycystic ovarian syndrome. Proc. Natl. Acad. Sci. (PNAS) U.S.A 112.2, 596-601
- 16. AM Moore, Prescott, M. Campbell, RE. 2013. Investigating estradiol negative and positive feedback in a prenatal androgen-induced mouse model of polycystic ovarian syndrome. Endocrinology 154, 796-806.