Dr. Sanjaya Abeysirigunawardena: "Modified nucleotides in ribosomal RNA and modification enzymes influence ribosomal assembly" | Kent State University

Dr. Sanjaya Abeysirigunawardena: "Modified nucleotides in ribosomal RNA and modification enzymes influence ribosomal assembly"

RNA-protein interactions are essential for many critical cellular processes such as post-transcriptional gene regulation and protein biosynthesis. In our laboratory we study the biophysics of RNA-Protein interactions. In particular we are investigating how various nucleotide modifications found in ribosomal RNA modulate RNA-protein interactions during the bacterial ribosome assembly. Synthesis of new ribosomes is critical for the survival of all organisms. In the early 1960s, Nomura and colleagues found that the 30S ribosomal subunit can be assembled in vitro and the ribosomal proteins are added hierarchically and cooperatively. Interestingly however, both assembly of the ribosome and post-transcriptional modification of rRNA occur simultaneously during ribosome biogenesis in vivo. Unfortunately, the interrelationship of these two processes is not well studied. Our goal is to understand how post‐transcriptional RNA modifications in 16S rRNA and their corresponding modification enzymes influence 30S ribosome assembly. For example, modification enzymes can stabilize the binding of ribosomal proteins to productive pre-30S complexes (thermodynamic cooperativity) as shown in the top panel of figure 1. Similarly, due to “kinetic cooperativity”, modification enzymes can prevent formation of unproductive intermediate complexes by blocking the binding site of certain ribosomal proteins. On the other hand, structural and stability changes caused by modified ribonucleotides can favor the binding of ribosomal proteins (Figure 1, bottom panel). We use various biophysical methods including single-molecule fluorescence measurements to investigate the preferred pathways of 30S assembly in the presence of the nucleotide modifications and modification enzymes.

References

  1. Mizushima, S, Nomura, M. (1970) Assembly mapping of 30S ribosomal proteins from E. coli. Nature 226:1214–18.
  2. Williamson J.R. (2008) Cooperativity in macromolecular assembly. Nat Chem Biol. 4:458-65.
  3. Kim, H., Abeysirigunawardena, S.C., Chen, K., Mayerle, M., Ragunathan, K., Luthey-Schulten, Z., Ha, T., Woodson, S.A. (2014) Protein-guided RNA dynamics during early ribosome assembly. Nature 506:334-8.
  4. Chow, C.S., Lamichhane, T.N., Mahto, S.K. (2007) Expanding the nucleotide repertoire of the ribosome with post-transcriptional modifications. ACS Chem Biol. 2:610-9.
  5. Abeysirigunawardena, S.C., Chow, C.S. (2008) pH-dependent structural changes of helix 69 from Escherichia coli 23S ribosomal RNA. RNA 14:782-92.
  6. Popova, A.M., Williamson, J.R. (2014) Quantitative analysis of rRNA modifications using stable isotope labeling and mass spectrometry. J Am Chem Soc. 5:2058-69.