Our research interests in a broad sense are to understand the biological functions of certain proteins from their structural point of views. Particularly, we focus on structure-function relationships of metalloenzymes, as well as protein-protein interaction and protein-ligand interactions that are related to signal transductions in cells. Our long-term goal is to translate these basic research studies into the discovery of new drugs and therapeutic methods to treat human diseases.
Our research interests are in the following areas:
Regulation of Nitric Oxide Synthesis in Bacterial Nitric Oxide Synthase (NOS)
Receptor and Protein Interactions in NO Signaling
Structure-function Studies of Heme O2 and NO Sensors
Mechanism of NOS-related Endothelial Dysfunction
Scholarly, Creative & Professional Activities
Tejero, J., Biswas, A., Haque, M.M., Wang, Z.-Q., Hemann, C., Varnado, C.L., Novince, Z., Hille, R., Goodwin. D.C., Stuehr, D.J. “Mesohaem substitution reveals how haem electronic properties can influence the kinetic and catalytic parameters of neuronal NO synthase.” Biochem. J. 433, 163-74, 2010.
Wang, Z.-Q., Wei, C.-C., and Stuehr, D. J. “How Does A Valine Residue That Modulates Heme-NO Binding Kinetics in Inducible NO Synthase Regulate Enzyme Catalysis” J Inorg. Biochem. 104, 349-56, 2009.
Wei, C.-C., Wang, Z.-Q., Tejero J, Yang YP, Hemann C, Hille R and Stuehr D. J. “Catalytic reduction of a tetrahydrobiopterin radical within nitric-oxide synthase.” J. Biol. Chem., 283, 11734-42, 2008.
Wang, Z.-Q., Lawson R. J., Madhavan, B. R., Wei, C.-C., Crane, B. R., Munro, A.W., and Stuehr, D. J. “Bacterial Flavodoxin Support Nitric Oxide Production by Bacillus Subtilis Nitric-Oxide Synthase.” J. Biol. Chem., 282, 2196-202, 2007.
Wang, Z.-Q., Wei, C.-C., Santolini, J., Koustubh, P., Wang, Q. and Stuehr, D. J. “A Tryptophan That Modulates Tetrahydrobiopterin-Dependent Electron Transfer in Nitric Oxide Synthase Regulates Enzyme Catalysis by Additional Mechanisms.” Biochemistry, 44, 4676-90, 2005.
Wang, Z.-Q., Wei, C.-C., Sharma, M., Pant, K., Crane, B. R., and Stuehr, D. J. “A Conserved Val to Ile Switch Near the Heme Pocket of Animal and Bacterial Nitric-Oxide Synthases Helps Determine Their Distinct Catalytic Profiles. J. Biol. Chem., 279, 19018-25, 2004.
Wang, Z.-Q., and Stuehr D. J. “Calcium Signaling: NO Synthase”, Encyclopedia of Biological Chemistry, Edited by William J Lennarz, Deniel M lane, Academic Press, 256-260, Sep 2004.
Wei, C.-C., Wang, Z.-Q., Arvai A. S., Hemann, C., Hille, R., Getzoff E. D., and Stuehr, D. J. “Structure of Tetrahydrobiopterin Tunes Its Electron Transfer to the Heme-dioxy Intermediate in Nitric oxide synthase.” Biochemistry, 42, 1969-77, 2003.
Cao, C.-Y., Zhang, Q., Wang, Z.-Q., Wang, Y.-H., Wu, H.-M., and Huang, Z.-X. “1H-NMR Studies of the Effect in Heme of Cytochrome b5 Caused by Valine45 Site-directed Mutation.” Biochemie, 85, 1007-1016, 2003.
Wang, Z.-Q., Wei, C.-C., and Stuehr, D.J. "A Conserved Tryptophan 457 Modulates the Kinetics and Extent of N-hydroxy-L-Arginine Oxidation by Inducible Nitric Oxide Synthase” J. Biol. Chem., 277, 12830, 2002.
Wang, Z.-Q., Wei, C.-C., Ghosh S, Meade, A. L., Hemann, C., Hille, R., and Stuehr D. J. "A Conserved Trytophan In Nitric Oxide Synthesis Regulates Heme-dioxy Reduction by Tetrahydrobiopterin" Biochemistry, 40(43), 12819-25, 2001.
Wang, Z.-Q., Wang Y.-H., Wang W.-H., Xue, L.-L., Wu, X.-Z., Xie, Y., and Huang Z.-X., "The Effect of Mutation at Valine-45 on the Stability and Redox Potentials of Trypsin-cleaved Cytochrome b5", Biophysical Chemistry, 83(1), 3-17, 2000.
Wang, Z.-Q., Wang Y.-H., Qian, W., Wang H.-H., Chunyu, L.-J., Xie, Y., and Huang Z.-X., "Methanol-induced Unfolding and Refolding of Cytochrome b5 and Its P40V Mutant Monitored by UV-visible, CD and Fluorescence Spectra", J. Protein Chem., 18(5), 547-55, 1999.
Zhu B., Wang Z.-Q., Li, X.-M., Zhao D.-Q., and Ni, J.-Z., “Lanthanide Metal Complexes for the Hydrolysis of Ribonucleoside 3’,5’-cyclic Phosphate and Deoxyribonucleoside 3’, 5’-cyclic Phosphate”, Journal of Molecular Catalysis A-Chemical, 118(1). L5-L7, 1997.
Wang, Z.-Q., Zhang S.-G. Liu, Q.-M., and Ni, J.-Z., "Studies on The Action of Rare Earth with Bovine Serum Albumin in Multimolecular System", Journal of Rare Earth (Special Issue), 573, 1995.