CRISPR-Cas9

CRISPR-Cas9 has emerged as a potent genome editing tool that promises unprecedented control and specificity in targeting desired sequences and has been successfully employed in many cell lines and organisms. However, whether CRISPR-Cas9 can function in a setting where the target sequences can fold into stable secondary structures (alternative structures to the well-known double-helix DNA) is not known. In this project, researchers from Physics and Chemistry departments will establish capabilities and limitations of CRISPR-Cas9 in targeting such sequences and functioning in the vicinity of...